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Cyclin D1 antibody

This Mouse Monoclonal antibody specifically detects Cyclin D1 in WB, IHC, ELISA, FACS, IP, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.
Catalog No. ABIN574432

Quick Overview for Cyclin D1 antibody (ABIN574432)

Target

See all Cyclin D1 (CCND1) Antibodies
Cyclin D1 (CCND1)

Reactivity

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Human, Rat

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Cyclin D1 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

CD1-1
  • Brand

    IHC-plus™

    Specificity

    The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kD protein that migrates as a 36 kD band under reducing SDS-PAGE conditions.

    Purification

    Protein A purified

    Immunogen

    Purified cyclin D1 protein.

    Isotype

    IgG1
  • Application Notes

    Approved: ELISA, Flo, ICC (1 μg/mL), IHC, IHC-Fr (2 μg/mL), IHC-P (10 μg/mL), IP, WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 15 mM sodium azide,  pH 7.4

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    avoid freeze thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Target

    Cyclin D1 (CCND1)

    Alternative Name

    CCND1 / Cyclin D1

    Background

    Name/Gene ID: CCND1

    Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

    Gene ID

    595

    UniProt

    P24385

    Pathways

    PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
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